Biomolecular resource utilization in elementary cell-free gene circuits

摘要

We present a detailed dynamical model of the behavior oftranscription-translation circuits in vitro that makes explicit the rolesplayed by essential molecular resources. A set of simple two-gene test circuitsoperating in a cell-free biochemical 'breadboard' validate this model andhighlight the consequences of limited resource availability. In particular, weare able to confirm the existence of biomolecular 'crosstalk' and isolate itsindividual sources. The implications of crosstalk for biomolecular circuitdesign and function are discussed.